The fourth-generation biological tissue solidification technology has passed the acceptance of the expert group of the Ministry of Education. Its polymer replacement process can completely preserve the microstructure of biological organs at room temperature. This technology not only makes the muscle texture clarity of anatomical specimens meet the requirements of medical teaching, but also completely eliminates the toxic gas hazards of the traditional formalin immersion method. Many natural history museums have applied this technology to the production of rare species specimens, providing safer observation teaching aids for primary and secondary school biology classes.